il-1b (rabbit polyclonal antibody)  (Thermo Fisher)

Journal: Scientific Reports

Article Title: Network pharmacological prediction of the mechanism of action of Shen-Zhu-Lian-Bai Decoction in the treatment of ulcerative colitis

doi: 10.1038/s41598-024-64683-4

Figure Lengend Snippet: Primer Sequence.

Article Snippet: Afterwards, the membranes were incubated overnight at 4 °C with IL-6, IL-1B, and AKT1 rabbit polyclonal antibodies (sourced respectively from ZEN BIO, proteintech, and ZEN BIO).

Techniques: Sequencing

Journal: Scientific Reports

Article Title: Network pharmacological prediction of the mechanism of action of Shen-Zhu-Lian-Bai Decoction in the treatment of ulcerative colitis

doi: 10.1038/s41598-024-64683-4

Figure Lengend Snippet: Expression of IL-6, IL-1B and AKT1 between normal and UC samples. ** p < 0.01,**** p < 0.001.

Article Snippet: Afterwards, the membranes were incubated overnight at 4 °C with IL-6, IL-1B, and AKT1 rabbit polyclonal antibodies (sourced respectively from ZEN BIO, proteintech, and ZEN BIO).

Techniques: Expressing

Journal: Scientific Reports

Article Title: Network pharmacological prediction of the mechanism of action of Shen-Zhu-Lian-Bai Decoction in the treatment of ulcerative colitis

doi: 10.1038/s41598-024-64683-4

Figure Lengend Snippet: Molecular docking of quercetin (MOL000098) with its targets. ( A ) IL-6 protein-quercetin; ( B ) IL-1B protein-quercetin; ( C ) AKT1 protein-quercetin.

Article Snippet: Afterwards, the membranes were incubated overnight at 4 °C with IL-6, IL-1B, and AKT1 rabbit polyclonal antibodies (sourced respectively from ZEN BIO, proteintech, and ZEN BIO).

Techniques:

Journal: Scientific Reports

Article Title: Network pharmacological prediction of the mechanism of action of Shen-Zhu-Lian-Bai Decoction in the treatment of ulcerative colitis

doi: 10.1038/s41598-024-64683-4

Figure Lengend Snippet: Quercetin improves LPS-induced inflammatory damage of HCoEpiC cells. ( A ) The choice of LPS safety and effective concentration; ( B ) The choice of quercetin safety and effective concentration; ( C ) Levels of mRNA expression of IL-6; ( D ) Levels of mRNA expression of IL-1B; ( E ) Levels of mRNA expression of AKT1; ( F – I ) Expression of IL-6, IL-1B and AKT1 proteins. * p < 0.05,** p < 0.01,*** p < 0.001.

Article Snippet: Afterwards, the membranes were incubated overnight at 4 °C with IL-6, IL-1B, and AKT1 rabbit polyclonal antibodies (sourced respectively from ZEN BIO, proteintech, and ZEN BIO).

Techniques: Concentration Assay, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Imbalanced IL-1B and IL-18 Expression in Sézary Syndrome

doi: 10.3390/ijms24054674

Figure Lengend Snippet: Increased protein levels of epidermal IL-1B and dermal IL-18 in skin of erythrodermic SS. The expression of IL-1B and IL-18 ( A ) were assessed in the skin biopsies of SS patients ( n = 7–8, closed circle), healthy donors (HDs, n = 9–12, open circle), and idiopathic erythroderma (IE, n = 9–13, open triangle) by immunohistochemistry. The analyses were performed on the epidermis ( B ) and dermis ( C ). The values are expressed as medians. * p ≤ 0.05 and ** p ≤ 0.01.

Article Snippet: The tissue sections were then incubated with the primary antibodies polyclonal anti-NLRP1 rabbit antibody (Ab 3683); monoclonal mice anti-NLRP3 (214185); polyclonal anti-AIM2 rabbit antibody (93015); polyclonal anti-IL-18 rabbit antibody (Ab71495); and polyclonal anti-IL-1B rabbit antibody (Ab2105), all of which were from Abcam (Cambridge, MA, USA).

Techniques: Expressing, Immunohistochemistry

Journal: International Journal of Molecular Sciences

Article Title: Imbalanced IL-1B and IL-18 Expression in Sézary Syndrome

doi: 10.3390/ijms24054674

Figure Lengend Snippet: Decreased IL-1B expression and IL-18 upregulation in lymph nodes at an advanced stage of SS. The expressions of IL-1B and IL-18 were assessed in the lymph-node biopsies of SS patients at stages N1 ( n = 4, open circle), N2 ( n = 2, pink circle), and N3 ( n = 2, closed circle), as well as in idiopathic erythroderma patients (IE, n = 8, open triangle), by immunohistochemistry. ( A ) Representative images, original magnification ×20. ( B ) IL-1B expression and IL-18 expression. Data are illustrated by median values. * p ≤ 0.05.

Article Snippet: The tissue sections were then incubated with the primary antibodies polyclonal anti-NLRP1 rabbit antibody (Ab 3683); monoclonal mice anti-NLRP3 (214185); polyclonal anti-AIM2 rabbit antibody (93015); polyclonal anti-IL-18 rabbit antibody (Ab71495); and polyclonal anti-IL-1B rabbit antibody (Ab2105), all of which were from Abcam (Cambridge, MA, USA).

Techniques: Expressing, Immunohistochemistry

Journal: International Journal of Molecular Sciences

Article Title: Imbalanced IL-1B and IL-18 Expression in Sézary Syndrome

doi: 10.3390/ijms24054674

Figure Lengend Snippet: Imbalanced expression of IL-1B and IL-18, as well as their associated regulators of inflammation, in Sézary syndrome.

Article Snippet: The tissue sections were then incubated with the primary antibodies polyclonal anti-NLRP1 rabbit antibody (Ab 3683); monoclonal mice anti-NLRP3 (214185); polyclonal anti-AIM2 rabbit antibody (93015); polyclonal anti-IL-18 rabbit antibody (Ab71495); and polyclonal anti-IL-1B rabbit antibody (Ab2105), all of which were from Abcam (Cambridge, MA, USA).

Techniques: Expressing